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Figure 31 . Ice cube 14S Computer Controlled freezing system with integrated PC. Capacity: 1,080 straws (0.25 ml & 0.5 ml straws). (Courtesy of Minitube International). Martin Hilber, Adriane, Chersich Matthew F., van de Wijgert Janneke H.H.M., Rees Helen, Temmerman Marleen. 2007. “ Vaginal Practices, Microbicides and HIV: What Do We Need to Know?” Sexually Transmitted Infections 83 ( 7): 503–505. doi: 10.1136/sti.2007.027748. [ PMC free article] [ PubMed] [ CrossRef] [ Google Scholar] Explain the need for a chaperone: “One of the female ward staff members will be present throughout the examination, acting as a chaperone, would that be ok?”

Go slowly! Don’t force it in if you feel pressure or rush the toy through the urethra. Some heavy toys, like steel or titanium plugs, slide in on their own. There are different kinds of electroejaculators available in the market. They may be battery operated or plugged into an electrical outlet. They may be manually operated, operate from a built in program and may be programmable; some have all three options. Most bulls ejaculate with electrical stimuli of < 9 volts. There are also different kinds of rectal probes that vary in diameter, weight, orientation of electrodes and in the number of electrodes. All modern probes have three ventrally oriented electrodes. Most probes have a U-shaped extension that fits around the bull's tail when the probe is inside the rectum. The bull’s tail keeps the probe oriented correctly. To obtain the best results with each individual bull it is important to choose the right probe size since its weight and diameter influence transmission of the stimulus to the nerves. Probes with larger diameter produce a stronger response to stimuli of a given electrical output than smaller diameter probes. Before loading the AI gun, pre-warm it, particularly if the environment is cold. This can be done by stroking it vigorously with clean paper towel, placing it close to your body for a few minutes in advance or using a purpose designed AI gun warmer unit.

Low Nicola, Chersich Matthew F., Schmidlin Kurt, Egger Matthias, Francis Suzanna C., van de Wijgert Janneke H.H.M., Hayes Richard J., et al. Figure 25 . Micropipettor with a handling wheel for filling individual straws. (Courtesy of Minitube International). Figure 11 . A bovine semen smear stained with eosin-nigrosin at 1000 X magnification. The white cells are live sperms (6 cells) and the red ones are dead sperms (1 cell). Semen Concentration

After filling, the straws may be sealed manually with heat sealers or ultrasonic sealers ( Fig. 26 ). The high-vaginal charcoal media swab is used to detect bacterial vaginosis, trichomonas vaginalis, candida and Group B streptococcus. Inspect for erosions around the os: most commonly associated with ectropion, however, early cervical cancer can have a similar appearance. Dilute the semen slowly with a small volume of warm Fraction A extender in a 50 ml dilution tube. Depending on the calculated number of straws to be processed, the ejaculate will be then extended with Fraction A to 50% of the calculated final volume. Fraction A extender temperature and the semen temperature should be the same (aprox. 28-32ºC) ( Fig. 18 ).


Smit J, Chersich MF, Beksinska M, Kunene B, Manzini N, Martin Hilber A, and Scorgie F. 2011. “ Prevalence and Self-Reported Health Consequences of Vaginal Practices in KwaZulu-Natal, South Africa: Findings from a Household Survey.” Tropical Medicine and International Health 16 ( 2): 245–256. doi: 10.1111/j.1365-3156.2010.02687.x. [ PubMed] [ CrossRef] [ Google Scholar] Zembe Yanga Z., Townsend Loraine, Thorson Anna, Ekström Anna, Ankomah A, Leclerc-Madlala S, Norris AH, et al. Apply a lot of lube near the urethral opening and to your toy. Use a water-based, chemical-free lube.

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